DNA Length Dependence of the Single-Strand Annealing Pathway and the Role of Saccharomyces cerevisiae RAD59 in Double-Strand Break Repair
نویسندگان
چکیده
منابع مشابه
Genetic requirements for the single-strand annealing pathway of double-strand break repair in Saccharomyces cerevisiae.
HO endonuclease-induced double-strand breaks (DSBs) within a direct duplication of Escherichia coli lacZ genes are repaired either by gene conversion or by single-strand annealing (SSA), with > 80% being SSA. Previously it was demonstrated that the RAD52 gene is required for DSB-induced SSA. In the present study, the effects of other genes belonging to the RAD52 epistasis group were analyzed. W...
متن کاملSaccharomyces cerevisiae Sin3p facilitates DNA double-strand break repair.
There are two main pathways in eukaryotic cells for the repair of DNA double-strand breaks: homologous recombination and nonhomologous end joining. Because eukaryotic genomes are packaged in chromatin, these pathways are likely to require the modulation of chromatin structure. One way to achieve this is by the acetylation of lysine residues on the N-terminal tails of histones. Here we demonstra...
متن کاملThe study of dose gamma rays of 192Ir source on DNA single strand break (SSB) and DNA double strand break (DSB) in soft tissue phantom
Introduction: Passage of ionizing radiation through the organs of living creatures develops clusters of damaged nucleotides inside the DNA rounds. 192Ir Gamma source is one of the most widely used sources in brachytherapy of cervical and prostate cancer. Thus, in this research, we investigated the flux of photons and its resulting secondary electrons, the single-strand break (S...
متن کاملThe Role of Long Non Coding RNAs in the Repair of DNA Double Strand Breaks
DNA double strand breaks (DSBs) are abrasions caused in both strands of the DNA duplex following exposure to both exogenous and endogenous conditions. Such abrasions have deleterious effect in cells leading to genome rearrangements and cell death. A number of repair systems including homologous recombination (HR) and non-homologous end-joining (NHEJ) have been evolved to minimize the fatal effe...
متن کاملRole of Saccharomyces Single-Stranded DNA-Binding Protein RPA in the Strand Invasion Step of Double-Strand Break Repair
The single-stranded DNA (ssDNA)-binding protein replication protein A (RPA) is essential for both DNA replication and recombination. Chromatin immunoprecipitation techniques were used to visualize the kinetics and extent of RPA binding following induction of a double-strand break (DSB) and during its repair by homologous recombination in yeast. RPA assembles at the HO endonuclease-cut MAT locus...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 2000
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.20.14.5300-5309.2000